(KO Validated) Caspase-3 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-60646-60, E-AB-60646-120, E-AB-60646-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: Caspase-3

Target Synonym: CPP32; CPP32B; SCA-1; Active Caspase 3; CASP3; active Caspase-3; Caspase 3; Caspase-3 p12; caspase-3

Research Areas: Cancer, Cell Biology, Metabolism, Neuroscience

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB, IHC

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P42574

Background: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human Caspase-3 (NP_004337.2).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000 IHC 1:50-1:200

Calculated MW: 31kDa

Observed MW: 17kDa/35kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only