CD126 Recombinant Protein

Catalogue Number: NCP0257-500, NCP0257-1000

Sizes: 500ug, 1mg

Swiss-Prot: P08887

Host: E.coli

Tag: His-tag

AA Sequence: CTGGCTCCTCGTCGCTGTCCGGCCCAGGAAGTTGCCCGTGGTGTGCTGACCAGCCTGCCGGGTGATAGCGTTACCCTGACCTGCCCGGGCGTTGAACCGGAAGATAATGCAACCGTTCATTGGGTGCTGCGTAAACCGGCCGCAGGCAGCCATCCGAGCCGTTGGGCTGGTATGGGTCGCCGTCTGCTGCTGCGCAGCGTGCAGCTGCATGATAGCGGCAATTATAGTTGCTATCGTGCCGGTCGCCCGGCAGGCACCGTTCATCTGCTGGTGGATGTGCCGCCGGAAGAACCGCAGCTGAGCTGCTTCCGCAAAAGCCCGCTGAGTAATGTGGTGTGCGAATGGGGCCCGCGTAGTACCCCGAGTCTGACCACCAAAGCCGTTCTGCTGGTGCGCAAATTCCAGAATAGCCCGGCCGAAGACTTCCAGGAACCGTGCCAGTATAGTCAGGAAAGTCAGAAATTCAGTTGCCAGCTGGCAGTGCCGGAAGGCGATAGCAGCTTCTATATTGTGAGTATGTGTGTTGCCAGCAGCGTTGGTAGCAAATTCAGTAAAACACAAACCTTCCAGGGTTGTGGCATTCTGCAGCCGGATCCGCCGGCCAATATTACCGTTACCGCAGTTGCCCGCAATCCGCGCTGGCTGAGTGTGACCTGGCAGGATCCGCATAGCTGGAATAGCAGCTTCTACCGCCTGCGCTTCGAACTGCGTTATCGCGCAGAACGCAGTAAAACCTTCACCACCTGGATGGTTAAAGATCTGCAGCATCATTGCGTGATTCATGATGCATGGAGTGGCCTGCGTCATGTGGTTCAGCTGCGCGCACAGGAAGAATTCGGCCAGGGTGAATGGAGTGAATGGAGCCCGGAAGCAATGGGCACCCCGTGGACCGAAAGCCGCAGCCCTCCGGCTGAAAATGAAGTGAGCACCCCGATGCAGGCACTGACCACCAATAAAGATGATGATAATATTCTGTTCCGCGATAGCGCCAATGCCACCAGTCTGCCGGTTCAGGATAGTAGCAGCGTGCCGCTGCCG

Restriction Sites: NdeI-XhoI

Background: The IL-6 receptor is a heterodimeric complex that consists of a ligand-binding IL-6 receptor α (IL-6Rα) subunit and a signaling component, gp130. Binding of IL-6 to IL-6Rα results in dimerization of receptor with gp130 and subsequent STAT3 activation (1). IL-6Rα is cleaved from the cell surface by ADAM17. In humans, soluble IL-6Rα is also generated via alternatively spliced mRNA. Soluble IL-6Rα binds to IL-6 and can stimulate signaling via membrane bound gp130 in a process known as “trans-signaling”. It is through trans-signaling that IL-6 stimulates cells that do not express membrane bound IL-6Rα.

Soluble: PBS, 4M Urea, PH7.4

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Expression Vector: pet-22b(+)

BioWorld Molecular Weight: ~44kDa

Notes: For research use only, not for use in diagnostic procedure.